کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1934238 1050635 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Transcriptional activation of Cidec by PPARγ2 in adipocyte
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Transcriptional activation of Cidec by PPARγ2 in adipocyte
چکیده انگلیسی

Cidec is a lipid droplet-associated protein, which inhibits lipolysis, leading to the accumulation of triglycerides in adipocytes. However, the transcriptional regulation of Cidec in adipocyte remains unknown. In the present study we investigated that the mouse Cidec transcript is regulated by PPARγ2. After the differentiation of adipocyte, the expression pattern of Cidec was similar to that of PPARγ2. In the presence of a PPARγ agonist, the level of Cidec mRNA was highly increased. In addition, putative PPRE sites were identified in the Cidec promoter. By chromatin immunoprecipitation assay and reporter assay, we observed the binding of PPARγ2 to the promoter of Cidec. Gel shift assay and the mutagenesis study were showed that the −219/−207 region of the Cidec promoter could function as a PPRE of the Cidec promoter. These results suggest that PPARγ2 is required for the transcriptional activity of Cidec during adipogenesis, which could be contributed to understand the molecular mechanism of lipid droplet formation in adipocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 377, Issue 1, 5 December 2008, Pages 297–302
نویسندگان
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