کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1934243 | 1050635 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: IgE-induced degranulation of mucosal mast cells is negatively regulated via nicotinic acetylcholine receptors IgE-induced degranulation of mucosal mast cells is negatively regulated via nicotinic acetylcholine receptors](/preview/png/1934243.png)
The autonomic nervous system is known to mediate mast cell activation. We investigated expression of nicotinic acetylcholine receptors (nAChRs) in mucosal-type mast cells and their contribution to the regulation of mast cell activation. Expression of mRNA of nAChR α4, α7, and β2 subunits were detected in specially differentiated mucosal-type murine bone marrow-derived mast cells (mBMMCs). Pretreatment with non-specific nAChRs agonists, acetylcholine, nicotine and epibatidine and a specific α7 subunit agonist GTS-21 significantly inhibited antigen-induced degranulation of mBMMCs in a dose-dependent manner and GTS-21-induced inhibition was significantly blocked by α7 subunit antagonist, α-bungarotoxin. Furthermore, confocal microscopy also demonstrated surface binding of α-bungarotoxin on mBMMCs. Our findings indicate that mucosal mast cell activation may be negatively regulated mainly through nAChR α7 subunit, suggesting that nAChRs are involved in neuronal-mucosal mast cell interactions.
Journal: Biochemical and Biophysical Research Communications - Volume 377, Issue 1, 5 December 2008, Pages 321–325