کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1934361 | 1050638 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Inorganic phosphate regulates Glvr-1 and -2 expression: Role of calcium and ERK1/2 Inorganic phosphate regulates Glvr-1 and -2 expression: Role of calcium and ERK1/2](/preview/png/1934361.png)
Sodium-dependent phosphate cotransporters are key regulators of phosphate homeostasis and play a major role in mineralized tissues remodelling. However, factors influencing their expression remain under consideration. In our study, modulation of type III sodium-dependent phosphate cotransporters expression by inorganic phosphate (Pi) was investigated in the murine odontoblast-like cell line MO6-G3. Experiments were designed to determine the effects of phosphate release on dental cells during tooth decay. By real-time RT-PCR we demonstrated that Glvr-1 and -2 expressions are up-regulated by Pi. The increase in Glvr-1 and -2 expressions was correlated with ERK1/2 phosphorylation and calcium/phosphate crystals formation in cultured wells. Using calcium-free culture conditions or the specific inhibitor of ERK phosphorylation (UO126), we demonstrated that Pi effects on Glvr-1 and -2 up-regulation require the presence of calcium and involve ERK signalling pathways. This study contributes to give new insights in the control of Pi transport during carious diseases.
Journal: Biochemical and Biophysical Research Communications - Volume 381, Issue 2, 3 April 2009, Pages 259–263