کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1934546 | 1050644 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The phosphatase inhibitor, okadaic acid, strongly protects primary rat cortical neurons from lethal oxygen–glucose deprivation The phosphatase inhibitor, okadaic acid, strongly protects primary rat cortical neurons from lethal oxygen–glucose deprivation](/preview/png/1934546.png)
The protein kinase-mediated actions of peptide growth factors such as IGF-1 and bFGF protect cultured neurons from being killed by the oxygen and glucose deprivations (OGD) that prevail in the ‘stroked brain’. Here, we show that neuroprotection by IGF-1 is mediated by PI-3K/Akt, whereas that of bFGF is mediated by MAPK. IGF-1 and bFGF together did not further increase protection suggesting a downstream convergence of their pathways. Since protein kinases mediated the protection, a phosphatase inhibitor such as okadaic acid (OA) might be as protective as the growth factors against OGD. Here, we show that OA is actually a much more effective protector. It increased the phosphorylation of both PI-3K/Akt and MAPK, and stimulated new protein synthesis. OA also acted independently of the CREB activation and FKHRL1 and GSK-3 inactivation which have been implicated in IGF-1 actions.
Journal: Biochemical and Biophysical Research Communications - Volume 378, Issue 3, 16 January 2009, Pages 394–398