کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1934768 1050650 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
N-Glycosylation modulates the cell-surface expression and catalytic activity of corin
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
N-Glycosylation modulates the cell-surface expression and catalytic activity of corin
چکیده انگلیسی

N-Glycosylation may influence the subcellular localization and biological activity of the pro-ANP convertase, corin. In HEK293–corin cells, the inhibition of N-glycosylation, with tunicamycin, reduced the cell-surface expression of murine corin, but did not alter the total expression. Therefore, tunicamycin treatment likely caused the intracellular accumulation of non-glycosylated corin. Tunicamycin treatment also significantly reduced corin activity (pro-ANP cleavage) in these cells. We developed an assay to measure the effect of N-glycosylation on corin activity, independent of its effect on corin localization. We determined that the reduction in corin activity was due to a direct effect of N-glycosylation, and was not secondary to the effect of N-glycosylation on corin cell-surface expression. Our data provide evidence that N-glycosylation is essential for the cell-surface expression of murine corin and modulates its functional activity. N-Glycosylation represents a possible mechanism for the regulation of native corin on the surface of cardiomyocytes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 373, Issue 1, 15 August 2008, Pages 130–135
نویسندگان
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