کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1934973 1050654 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inhibition of NF-κB signaling via tyrosine phosphorylation of Ymer
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Inhibition of NF-κB signaling via tyrosine phosphorylation of Ymer
چکیده انگلیسی

Cytoplasmic zinc finger protein A20 functionally dampens inflammatory signals and apoptosis via inhibition of NF-κB activation. We have reported that Ymer interacts with A20 and lysine (K)-63-linked polyubiquitin chain and that Ymer inhibits NF-κB signaling in collaboration with A20. It has also been reported that Ymer is phosphorylated by EGF stimulation. We found that Ymer was considerably phosphorylated on tyrosine residues also via Src family kinases such as Lck. A luciferase reporter assay showed that mutation of tyrosines on Ymer (YmerY217/279/304F) results in loss of the inhibitory activity for NF-κB signaling. Furthermore, a soft agar colony formation assay showed that the combination of SrcY527F and YmerY217/279/304F has no ability for anchorage-independent growth, suggesting that tyrosine phosphorylation of Ymer is important for inhibition of the NF-κB-mediated apoptotic pathway. These findings demonstrate that Ymer is likely to be a negative regulator for the NF-κB signaling pathway.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 378, Issue 4, 23 January 2009, Pages 744–749
نویسندگان
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