کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1934998 | 1050654 | 2009 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The lipid peroxidation metabolite 4-oxo-2-nonenal cross-links α-synuclein causing rapid formation of stable oligomers The lipid peroxidation metabolite 4-oxo-2-nonenal cross-links α-synuclein causing rapid formation of stable oligomers](/preview/png/1934998.png)
Recently, the aldehyde 4-oxo-2-nonenal (ONE) was identified as a product of lipid peroxidation and found to be an effective protein modifier. In this in vitro study we investigated structural implications of the interaction between ONE and α-synuclein, a protein which forms intraneuronal inclusions in neurodegenerative disorders such as Parkinson’s disease and dementia with Lewy bodies. Our results demonstrate that ONE induced an almost complete conversion of monomeric α-synuclein into 40–80 nm wide and 6–8 nm high soluble β-sheet-rich oligomers with a molecular weight of ∼2000 kDa. Furthermore, the ONE-induced α-synuclein oligomers displayed a high stability and were not sensitive to treatment with sodium dodecyl sulfate, indicating that ONE stabilized the oligomers by cross-linking individual α-synuclein molecules. Despite prolonged incubation the oligomers did not continue to aggregate into a fibrillar state, thus suggesting that these α-synuclein species were not on a fibrillogenic pathway.
Journal: Biochemical and Biophysical Research Communications - Volume 378, Issue 4, 23 January 2009, Pages 872–876