کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1935208 1050660 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Nephrocystin-1 interacts directly with Ack1 and is expressed in human collecting duct
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Nephrocystin-1 interacts directly with Ack1 and is expressed in human collecting duct
چکیده انگلیسی

Nephronophthisis is characterised by renal fibrosis, tubular basement membrane disruption and corticomedullary cyst formation leading to end stage renal failure. Mutations in NPHP1 account for the underlying genetic defect in 25% of patients with nephronophthisis. Loss of urine concentration ability may be an early feature of nephronophthisis. Using yeast-2-library screening with the SH3 domain of nephrocystin-1 as bait, we identify Ack1 as a novel interaction partner. This interaction is confirmed using exogenous over-expression followed by co-immunoprecipitation. Ack1 is an activated Cdc42-associated kinase, and like nephrocystin-1, is a known interactor of p130Cas. Nephrocystin-1 partially colocalises with Ack1 at cell–cell contacts in IMCD3 cells. In human kidney, nephrocystin-1 expression is limited to cell–cell junctions in renal collecting duct cells. These data define Ack1 as a novel interaction partner of nephrocystin-1 and implicate cell–cell junctions and the renal collecting duct in the pathology of nephronophthisis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 371, Issue 4, 11 July 2008, Pages 877–882
نویسندگان
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