کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1935254 | 1050661 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Methyltransferase-inhibition interferes with neuronal differentiation of P19 embryonal carcinoma cells
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کلمات کلیدی
GFAPAdoMetbHLHBMP-4AdoxAdenosine dialdehydeTuj1P19 embryonal carcinomaS-adenosylmethionine - اس-ادنوزیل متیونینbasic helix-loop-helix - اسلحه پایه حلقه ایCell–cell interaction - تعامل سلول-سلولNeuronal differentiation - تمایز نورونRetinoic acid - رتینوئیک اسیدSAHH - شفاGlial fibrillary acidic protein - پروتئین اسیدی فیبریلاسیون گلایالbone morphogenetic protein 4 - پروتئین مورفوژنیک استخوان 4
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
We have analyzed the importance of substrate methylation by S-adenosylmethionine-dependent methyltransferases for neuronal differentiation of P19 embryonal carcinoma cells. We show that treatment of cells with methyltransferase inhibitor adenosine dialdehyde (AdOx) interferes with neuronal differentiation. Retinoic acid (RA) and AdOx co-treated cells had a decreased number of neurites and a flattened morphology compared with cells differentiated by RA. Also, the amount of neuronal class III tubulin (Tuj1) decreased from 76% to 9.6% with AdOx-treatment. Gene expression levels of wnt-1, brn-2, neuroD, and mash-1 were also down-regulated by AdOx-treatment. But AdOx-treatment did not up-regulate BMP-4 and GFAP genes. Treatment of RA decreased E-cadherin expression during neuronal differentiation. However, in AdOx/RA co-treated cells, E-cadherin expression was restored to the control level. Also, mRNA expression of N-cadherin decreased with AdOx-treatment. Taken together, these data show that methylation reactions might influence the cell-fate decision and neuronal differentiation of P19 cells.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 377, Issue 3, 19 December 2008, Pages 935-940
Journal: Biochemical and Biophysical Research Communications - Volume 377, Issue 3, 19 December 2008, Pages 935-940
نویسندگان
Sukchul Hong, Jieun Heo, Sangjin Lee, Seok Heo, Sung-Soo Kim, Young-don Lee, Moosik Kwon, Sungyoul Hong,