کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1935289 | 1050662 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: UbcH6 interacts with and ubiquitinates the SCA1 gene product ataxin-1 UbcH6 interacts with and ubiquitinates the SCA1 gene product ataxin-1](/preview/png/1935289.png)
UbcH6 is a member of an evolutionally conserved subfamily of E2 ubiquitin-conjugating enzymes. In this study, we report that UbcH6 interacts with and ubiquitinates ataxin-1, the spinocerebellar ataxia type 1 gene product. UbcH6 was identified as an ataxin-1-interacting protein using a yeast two-hybrid screen. UbcH6 co-immunoprecipitates and co-localizes with the ataxin-1 protein in the nucleus. Our binding assays showed that ataxin-1 interacts with UbcH6 through its AXH domain. Interestingly, UbcH6 could ubiquitinate ataxin-1 in the absence of an E3 ligase. The expression level of UbcH6 regulated the rate of ataxin-1 degradation. This study demonstrates that UbcH6 and ataxin-1 are E2-substrate cognate pairs in the ubiquitin–proteasome system.
Journal: Biochemical and Biophysical Research Communications - Volume 371, Issue 2, 27 June 2008, Pages 256–260