کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1935344 1050663 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Construction and characterization of the HMGB1 mutant as a competitive antagonist to HMGB1 induced cytokines release
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Construction and characterization of the HMGB1 mutant as a competitive antagonist to HMGB1 induced cytokines release
چکیده انگلیسی

Recent studies indicate that the High Mobility Group Box-1 protein (HMGB1) acts as a potent proinflammatory cytokine that contributes to the pathogenesis of diverse inflammatory and infectious disorders. The proinflammatory cytokine activity of HMGB1 has become a therapeutic target. In this study, we cloned the cDNA encoding human HMGB1 and constructed HMGB1 mutants using a one-step opposite direction PCR. The binding of the HMGB1 mutants to THP-1 cell and the cytokine activities of these HMGB1 mutants were observed. Results showed that the HMGB1 Mut (102–105), one of the HMGB1 mutants, in which amino acids 102–105 (FFLF) were replaced with two Glys, significantly decreased the full-length HMGB1 protein induced TNF-α release in human monocyte cultures. The results indicate that we have developed a novel recombinant HMGB1 mutant that competitively antagonizes the proinflammatory activity of HMGB1. This may be of significant importance in providing a new anti-inflammatory strategy for the treatment of severe sepsis and other inflammatory disorders.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 372, Issue 4, 8 August 2008, Pages 703–707
نویسندگان
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