کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1935535 | 1050668 | 2008 | 4 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: DNase I footprinting of the nucleosome in whole nuclei DNase I footprinting of the nucleosome in whole nuclei](/preview/png/1935535.png)
DNase I was used to footprint the 147 bp DNA fragment of the nucleosome in whole chicken erythrocyte nuclei. It was found that the higher-order structure imposes an additional protection on nucleosomes at sites close to the entry and exit points of the linker DNA, around the dyad axis (site S 0). The observed protection is extended up to 20 bp on either side of S 0. It is partial (∼50%) and most probably reflects a full protection of different regions in alternatively oriented nucleosomes. These are the same regions which interact with linker histones. The results strongly support the findings by simulation of DNase I digests of unlabelled oligonucleosome fragments in the 30 nm fibre that in all nucleosomes sites S −5 to S −3 and S +3 to S +5 ara on the outside of the fibre exposed to DNase I.
Journal: Biochemical and Biophysical Research Communications - Volume 372, Issue 1, 18 July 2008, Pages 226–229