کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1935647 | 1050671 | 2008 | 6 صفحه PDF | دانلود رایگان |
We report for the first time a new RNA replication system with a hepatitis C virus (HCV) strain (AH1) derived from a patient with acute hepatitis C. Using an HCV replicon RNA library constructed with the AH1 strain (genotype 1b), we first established a cloned cell line, sAH1, harboring the HCV replicon. Cured cells obtained with interferon treatment of sAH1 cells were used for transfection with genome-length HCV RNA possessing four mutations found in sAH1 replicon. Consequently, one cloned cell line, AH1, supporting efficient replication of genome-length HCV RNA was obtained. By the comparison of AH1 cells with the O cells supporting genome-length HCV RNA (HCV-O strain) replication, we found different anti-HCV profiles of interferon-γ and cyclosporine A between AH1 and O cells. Reporter assay analysis suggests that the diverse effects of interferon-γ are due to the difference in HCV strains, but not the cellular environment.
Journal: Biochemical and Biophysical Research Communications - Volume 371, Issue 1, 20 June 2008, Pages 104–109