کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1935712 1050673 2008 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The novel gene AngRem104 downregulates glucocorticoid receptor expression and activates NF-κB in human mesangial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
The novel gene AngRem104 downregulates glucocorticoid receptor expression and activates NF-κB in human mesangial cells
چکیده انگلیسی
AngRem104 [angiotensin II (Ang II)-related genes in human mesangial cells (MCs), clone104], a novel gene in human MCs induced by Ang II, was previously identified in human MCs and found to interact with several proteins. The current study used a yeast two-hybrid system and co-immunoprecipitation to investigate the interaction between AngRem104 and glucocorticoid receptor (GR) AF-1-specific elongation factor (GR-EF). GR expression was downregulated and the number of MCs positive for activated nuclear factor κB (NF-κB) was increased when AngRem104 was overexpressed. Transfection with antisense AngRem104 vector resulted in the upregulation of GR protein and reduced numbers of MCs with activated NF-κB. These results indicate that the novel gene AngRem104 is involved in the in vivo regulation of GR expression and the activation of NF-κB through interaction with GR-EF in human MCs.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 369, Issue 4, 16 May 2008, Pages 1057-1060
نویسندگان
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