کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1935955 1050677 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Molecular basis of chromium insulin interactions
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Molecular basis of chromium insulin interactions
چکیده انگلیسی

The physiological role of chromium (III) in diabetes mellitus has been an area of inconclusive research for many years. It is of great interest to explore the interactions made by chromium (III) to get a better insight into their role in glucose metabolism. To understand the molecular basis of chromium action we have carried out spectroscopic and crystallographic investigations on the binding of Cr(III)-Salen with insulin, as Cr(III)-Salen is reported to result in the enhancement of insulin activity. The Cr(III)–insulin complex formation has been characterised at two pHs, viz., 3.5 and 9.0 using UV–Vis and fluorescence studies. The crystallographic analysis of Cr(III)-Salen soaked cubic insulin crystals, using anomalous difference Fourier method, revealed B21 Glu to be the binding site for chromium (III).

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 369, Issue 2, 2 May 2008, Pages 725–729
نویسندگان
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