کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
193608 459774 2009 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
New approach of constructing biosensing matrices by physical and chemical crosslinking of biotin-alginate with alginate-pyrrole
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
New approach of constructing biosensing matrices by physical and chemical crosslinking of biotin-alginate with alginate-pyrrole
چکیده انگلیسی

A new matrix was designed and constructed; enabling the physical and chemical co-crosslinking of alginate-biotin (Alg-B) with alginate-pyrrole (Alg-py), namely biotinylated-glucose oxidase (B-GOx) was conjugated to Alg-B through avidin (Av) bridges, thereafter mixed with Alg-Py. The alginate was physically crosslinked via calcium ions followed by pyrrole covalent crosslinking through electro-generation of polypyrrole networks. The pyrrole electropolymerization on the electrode surface created spatial wires which tightened the polymeric coating to the electrode surface, packed the 3D structure and reinforced its structure by the covalent bonds. These matrices exhibited better stability than those without Alg-Py even under vigorous stirring applied during the amperometric measurements. When the concentration of the glucose analyte was determined by hydrogen peroxide oxidation, the current was conducted faster to the electrode surface, therefore providing a low response time and higher sensitivity value; using a relatively low enzyme load.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochimica Acta - Volume 54, Issue 18, 15 July 2009, Pages 4359–4364
نویسندگان
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