کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1936107 | 1050683 | 2008 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy Generation of a transgenic rice seed-based edible vaccine against house dust mite allergy](/preview/png/1936107.png)
As an alternative approach to conventional allergen-specific immunotherapy, transgenic rice seed expressing a major house dust mite (HDM) allergen, Der p 1, was developed as an edible vaccine. The C-terminal KDEL-tagged Der p 1 allergen specifically accumulated in seed endosperm tissue under the control of the endosperm-specific GluB1 promoter. Der p 1 reached a maximum concentration of 58 μg/grain and was deposited in the endoplasmic reticulum (ER)-derived protein body I (PB-I). Plant-derived Der p 1 was posttranslationally modified with high-mannose-type glycan structures. Glycosylated Der p 1 displayed reduced IgE binding capacity in comparison with its unglycosylated counterpart in vitro. Our results indicate that transgenic Der p 1 rice seeds are a safe, potential oral delivery vaccine for the treatment of HDM allergy.
Journal: Biochemical and Biophysical Research Communications - Volume 365, Issue 2, 11 January 2008, Pages 334–339