کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1936204 | 1050685 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential regulation of Navβ subunits during myogenesis
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Voltage-gated sodium channels (Nav) consist of a pore-forming α subunit (Navα) associated with β regulatory subunits (Navβ). Adult skeletal myocytes primarily express Nav1.4 channels. We found, however, using neonatal L6E9 myocytes, that myofibers acquire a Nav1.5-cardiac-like phenotype efficiently. Differentiated myotubes elicited faster Nav1.5 currents than those recorded from myoblasts. Unlike myoblasts, INa recorded in myotubes exhibited an accumulation of inactivation after the application of trains of pulses, due to a slower recovery from inactivation. Since Navβ subunits modulate channel gating and pharmacology, the goal of the present work was to study Navβ subunits during myogenesis. All four Navβ (Navβ1-4) isoforms were present in L6E9 myocytes. While Navβ1-3 subunits were up-regulated by myogenesis, Navβ4 subunits were not. These results show that Navβ genes are strongly regulated during muscle differentiation and further support a physiological role for voltage-gated Na+ channels during development and myotube formation.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 368, Issue 3, 11 April 2008, Pages 761-766
Journal: Biochemical and Biophysical Research Communications - Volume 368, Issue 3, 11 April 2008, Pages 761-766
نویسندگان
Miren David, Ramón MartÃnez-Mármol, Teresa Gonzalez, Antonio Felipe, Carmen Valenzuela,