کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1936340 | 1050689 | 2007 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Molecular force spectroscopy of homophilic nectin-1 interactions
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Molecular force spectroscopy of homophilic nectin-1 interactions Molecular force spectroscopy of homophilic nectin-1 interactions](/preview/png/1936340.png)
چکیده انگلیسی
Nectins are Ca2+ independent cell adhesion molecules localizing at the cadherin based adherens junctions. In this study, we have used atomic force microscopy to study interaction of a chimera of extra cellular fragment of nectin-1 and Fc of human IgG (nef-1) with wild type L-fibroblasts that express endogenous nectin-1 to elucidate the biophysical characteristics of homophilic nectin-1 trans-interactions at the level of single molecule. Bond strength distribution revealed three distinct bound states (or configurations) of trans-interactions between paired nectins, where each bound state has a unique unstressed off-rate and reactive compliance. Kinetic analysis of force-dependent off-rate of the bound state involving trans-interacting V-V domains between paired nectin-1 (unstressed off-rate â¼1.465 ± 0.779 sâ1, reactive compliance â¼0.143 ± 0.072 nm) was found to be closest to E-cadherin, indicating that V-V domain trans-interactions are probably necessary to initiate and promote adhesions of E-cadherin at adherens junctions (AJs).
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 362, Issue 4, 3 November 2007, Pages 886-892
Journal: Biochemical and Biophysical Research Communications - Volume 362, Issue 4, 3 November 2007, Pages 886-892
نویسندگان
Sri Ram Krishna Vedula, Tong Seng Lim, Shi Hui, P. Jaya Kausalya, E. Birgitte Lane, Gunaretnam Rajagopal, Walter Hunziker, Chwee Teck Lim,