کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936389 1050690 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Developmentally synchronized expression of two Bombyx mori Piwi subfamily genes, SIWI and BmAGO3 in germ-line cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Developmentally synchronized expression of two Bombyx mori Piwi subfamily genes, SIWI and BmAGO3 in germ-line cells
چکیده انگلیسی

The Piwi subfamily proteins and their partner, Piwi-interacting RNAs (piRNAs), play an important roles in germ-line development and silencing of selfish DNA elements. To date, however, the developmental expression profiles of the Piwi subfamily genes are poorly known. In this study, we examined the expression profiles of two Bombyx mori Piwi subfamily genes, silkworm Piwi (SIWI) and BmAGO3, which are possible partners of Bombyx piRNA-like small RNAs we recently identified in B. mori germ-line cells. Quantitative reverse transcription-polymerase chain reaction analyses demonstrated that these two genes were abundantly expressed in the larval testis, pupal ovary, and adult eggs, suggesting that they might be involved in spermatogenesis and oogenesis in Bombyx. Notably, developmental expression patterns of SIWI and BmAGO3 were remarkably similar. Collectively, our results suggest that SIWI and BmAGO3 may cooperate in an unknown pathway during the development of B. mori germ-line cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 367, Issue 4, 21 March 2008, Pages 755–760
نویسندگان
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