کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936401 1050690 2008 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Production of human erythropoietin by chimeric chickens
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Production of human erythropoietin by chimeric chickens
چکیده انگلیسی

The use of transgenic avian allows cost effective and safe production of pharmaceutical proteins. Here, we report the successful production of chimeric chickens expressing human erythropoietin (hEpo) using a high-titer retroviral vector. The hEpo expressed by transgenic hens accumulated abundantly in egg white and had N- and O-linked carbohydrates. While attachment of terminal sialic acid and galactose was incomplete, portions of N- and O-linked carbohydrates were present. In vitro biological activity of egg white–hEpo was comparable to that produced by recombinant CHO cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 367, Issue 4, 21 March 2008, Pages 834–839
نویسندگان
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