کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936481 1050692 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Polo-like kinase 2 gene expression is regulated by the orphan nuclear receptor estrogen receptor-related receptor gamma (ERRγ)
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Polo-like kinase 2 gene expression is regulated by the orphan nuclear receptor estrogen receptor-related receptor gamma (ERRγ)
چکیده انگلیسی

Estrogen receptor-related receptor gamma (ERRγ) is a member of the nuclear receptor family of transcriptional activators. To date, the target genes and physiological functions of ERRγ are not well understood. In the current study, we identify that Plk2 is a novel target of ERRγ. Northern blot analysis showed that overexpression of ERRγ induced Plk2 expression in cancer cell lines. ERRγ activated the Plk2 gene promoter, and deletion and mutational analysis of the Plk2 promoter revealed that the ERRγ-response region is located between nucleotides (nt) −2327 and −2229 and −441 and −432 (relative to the transcriptional start site at +1). Electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) analysis demonstrated that ERRγ binds directly to the Plk2 promoter. Overexpression of ERRγ in the presence of the mitotic inhibitor nocodazole significantly decreased apoptosis, and induced S-phase cell cycle progression through the induction of Plk2 expression. Taken together, these results demonstrated that Plk2 is a novel target of ERRγ, and suggest that this interaction is crucial for cancer cell proliferation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 362, Issue 1, 12 October 2007, Pages 107–113
نویسندگان
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