کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1936515 | 1050693 | 2008 | 5 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Bone marrow long label-retaining cells reside in the sinusoidal hypoxic niche Bone marrow long label-retaining cells reside in the sinusoidal hypoxic niche](/preview/png/1936515.png)
In response to changing signals, quiescent hematopoietic stem cells (HSCs) can be induced to an activated cycling state and provide multi-lineage hematopoietic cells to the whole body via blood vessels. However, the precise localization of quiescent HSCs in bone marrow microenvironment is not fully characterized. Here, we performed whole-mount immunostaining of bone marrow and found that BrdU label-retaining cells (LRCs) definitively reside in the sinusoidal hypoxic zone distant from the “vascular niche”. Although LRCs expressed very low level of a well-known HSC marker, c-kit in normal circumstances, myeloablation by 5-FU treatment caused LRCs to abundantly express c-kit and proliferate actively. These results demonstrate that bone marrow LRCs reside in the sinusoidal hypoxic niche, and function as a regenerative cell pool of HSCs.
Journal: Biochemical and Biophysical Research Communications - Volume 366, Issue 2, 8 February 2008, Pages 335–339