کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936631 1050697 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Functional role of stromal interaction molecule 1 (STIM1) in vascular smooth muscle cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Functional role of stromal interaction molecule 1 (STIM1) in vascular smooth muscle cells
چکیده انگلیسی

We investigated the functional role of STIM1, a Ca2+ sensor in the endoplasmic reticulum (ER) that regulates store-operated Ca2+ entry (SOCE), in vascular smooth muscle cells (VSMCs). STIM1 was mainly localized at the ER and plasma membrane. The knockdown of STIM1 expression by small interfering (si) RNA drastically decreased SOCE. In contrast, an EF-hand mutant of STIM1, STIM1E87A, produced a marked increase in SOCE, which was abolished by co-transfection with siRNA to transient receptor potential canonical 1 (TRPC1). In addition, transfection with siRNA against STIM1 suppressed phosphorylation of cAMP-responsive element binding protein (CREB) and cell growth. These results suggest that STIM1 is an essential component of SOCE and that it is involved in VSMC proliferation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 361, Issue 4, 5 October 2007, Pages 934–940
نویسندگان
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