کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936646 1050697 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Crystal structure of scaffolding protein CheW from thermoanaerobacter tengcongensis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Crystal structure of scaffolding protein CheW from thermoanaerobacter tengcongensis
چکیده انگلیسی

The crystal structure of the scaffolding protein CheW from Thermoanaerobacter tengcongensis (TtCheW) is reported with a resolution at 2.2 Å using molecular replacement. Based on the crystal structure TmCheA P4–P5–TmCheW from Thermotoga maritime reported by others, we modeled the TmCheA P4–P5–TtCheW complex and predicted that TtCheW is involved in a hydrophobic interaction with CheA, similar to that for TmCheW. We also found that the conserved motif “NxxGxIxP” from CheW plays an important role in CheA binding. The coincidence of the reported mutation sites related to CheW–MCP binding, and the predicted protein interaction region within the TtCheW molecule, suggest that CheW–MCP binding sites lie in the groove-shaped area between TtCheW and the CheA P4 domain within the assembled model.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 361, Issue 4, 5 October 2007, Pages 1027–1032
نویسندگان
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