کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936915 1050705 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Minor contribution of an internal ribosome entry site in the 5′-UTR of ornithine decarboxylase mRNA on its translation
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Minor contribution of an internal ribosome entry site in the 5′-UTR of ornithine decarboxylase mRNA on its translation
چکیده انگلیسی

The mechanism of synthesis of ornithine decarboxylase (ODC) at the level of translation was studied using cell culture and cell-free systems. Synthesis of firefly luciferase (Fluc) from the second open reading frame (ORF) in a bicistronic construct transfected into FM3A and HeLa cells was enhanced by the presence of the 5′-untranslated region (5′-UTR) of ODC mRNA between the two ORFs. However, cotransfection of the gene encoding 2A protease inhibited the synthesis of Fluc. Synthesis of Fluc from the second cistron in the bicistronic mRNA in a cell-free system was not affected significantly by the 5′-UTR of ODC mRNA. Synthesis of ODC from ODC mRNA in a cell-free system was inhibited by 2A protease and cap analogue (m7GpppG). Rapamycin inhibited ODC synthesis by 40–50% at both the G1/S boundary and the G2/M phase. These results indicate that an IRES in the 5′-UTR of ODC mRNA does not function effectively.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 364, Issue 1, 7 December 2007, Pages 124–130
نویسندگان
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