کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1936948 1050706 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Autophagic or necrotic cell death in the absence of caspase and bcl-2 family members
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Autophagic or necrotic cell death in the absence of caspase and bcl-2 family members
چکیده انگلیسی

How is one to investigate autophagic or necrotic cell death in the absence of interference from the apoptosis machinery? In the protist Dictyostelium, a model for the study of these two cell death types, we previously showed that autophagic cell death does not require paracaspase, the only caspase family member in this organism. In this report, we prepared two distinct paracaspase- atg1- double mutants, and we used them to demonstrate that paracaspase is not required for necrotic cell death either. Also, in silico investigation showed that the genome of Dictyostelium harbored no detectable member of the bcl-2 family and no single BH3 domain-bearing molecules. Altogether, in this model system both autophagic and necrotic cell death could occur, and could be investigated, with no interference from the two main molecular families involved in apoptosis, the caspase and the bcl-2 families.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 363, Issue 3, 23 November 2007, Pages 536–541
نویسندگان
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