کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1936972 | 1050706 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Toxoplasma gondii possesses a receptor for activated C kinase ortholog Toxoplasma gondii possesses a receptor for activated C kinase ortholog](/preview/png/1936972.png)
Receptor for activated C kinase 1 (RACK1) has been implicated in multiple protein–protein interactions including functioning as a scaffolding protein for signaling molecules. We report the cloning and cellular localization of a RACK1 ortholog (TgRACK1) in the opportunistic pathogen Toxoplasma gondii. The full-length transcript possesses a predicted ORF of 966 bp and codes for a protein of ∼35 kDa molecular weight. Molecular analysis of TgRACK1 reveals the presence of seven WD40 repeat motifs. TgRACK1 was tagged with a FLAG epitope and stably expressed in RH parasites. FLAG-TgRACK1 localizes to the parasite cytoplasm and nucleus. Immunoprecipitation (IP) of FLAG-TgRACK1 from highly purified extracellular parasites followed by immunoblot analysis reveals an interaction between TgβCOP and FLAG-TgRACK1. This is the first demonstration of an interaction between a βCOP subunit and the RACK1 protein. This result is of interest given that a signaling event precedes protein secretion and parasite invasion.
Journal: Biochemical and Biophysical Research Communications - Volume 363, Issue 3, 23 November 2007, Pages 680–686