کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937000 1050706 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
In silico restriction landmark genome scanning analysis of Xanthomonas oryzae pathovar oryzae MAFF 311018
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
In silico restriction landmark genome scanning analysis of Xanthomonas oryzae pathovar oryzae MAFF 311018
چکیده انگلیسی

We have developed a restriction landmark genome scanning (RLGS) system in silico, involving two-dimensional electrophoretic analysis of DNA by computer simulation that is based on the availability of whole-genome sequences for specific organisms. We applied the technique to the analysis of the Xanthomonas oryzae pathovar oryzae (Xoo) MAFF 311018, which causes bacterial blight in rice. The coverage that was found to be achievable using RLGS in silico, as a percentage of the genomic regions that could be detected, ranged from 44.5% to 72.7% per image. However, this reached a value of 96.7% using four images that were obtained with different combinations of landmark restriction enzymes. Interestingly, the signal intensity of some of the specific spots obtained was significantly lower than that of other surrounding spots when MboI, which cleaves unmethylated 5′-GATC-3′ sites, was used. DNA gel blot analysis with both DNA adenine methylase (Dam)-sensitive and -insensitive isoschizomers (MboI and Sau3AI) revealed that Dam-mediated DNA adenine methylation had indeed occurred at these particular sites. These results suggest that a significant portion of the 5′-GATC-3′ sites within the Xoo genome is stably methylated by Dam.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 363, Issue 3, 23 November 2007, Pages 852–856
نویسندگان
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