کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1937092 | 1050708 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Position-specific incorporation of biotinylated non-natural amino acids into a protein in a cell-free translation system Position-specific incorporation of biotinylated non-natural amino acids into a protein in a cell-free translation system](/preview/png/1937092.png)
Biotinylation is useful for the detection, purification and immobilization of proteins. It is performed by chemical modification, although position-specific and quantitative biotinylation is rarely achieved. We developed a position-specific biotinylation method using biotinylated non-natural amino acids. We showed that biotinylated p-aminophenylalanine derivatives were incorporated into a protein more efficiently than biotinylated lysine derivatives in a cell-free translation system. In addition, the biotinylated p-aminophenylalanines overcame the serious position-dependency observed for biotinylated lysines. The present method will be useful for detection and purification of proteins along with comprehensive exploration of surface-exposed residues and oriented immobilization of proteins.
Journal: Biochemical and Biophysical Research Communications - Volume 361, Issue 3, 28 September 2007, Pages 794–799