کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937111 1050709 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Onconase action on tRNALys3, the primer for HIV-1 reverse transcription
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Onconase action on tRNALys3, the primer for HIV-1 reverse transcription
چکیده انگلیسی

Onconase, a cytotoxic and antitumor RNase inhibits viral replication in chronically HIV-1-infected human cells under sub lethal concentrations. Cellular tRNA has been implicated as the target for onconase action. We have recently shown that onconase cleaves selectively at GG residues in the UGG context in the variable loop and D-arm of the tRNA substrates. We therefore examined onconase cleavage specificity in in vitro transcribed tRNALys3, which is the primer for HIV-1 reverse transcription but does not have UGG anywhere in its sequence. Onconase was found to cleave tRNALys3 predominantly at the GG residues in the GGG triplet present in the variable loop. Mutations at this site did not effect onconase cleavages. Interestingly thus, onconase seems to cleave predominantly in the variable loop of tRNALys3 regardless of the sequence context implying possible contribution of even structural determinants for its selective cleavages.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 363, Issue 2, 16 November 2007, Pages 304–309
نویسندگان
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