کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937112 1050709 2007 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Azasterols impair Giardia lamblia proliferation and induces encystation
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Azasterols impair Giardia lamblia proliferation and induces encystation
چکیده انگلیسی

The effects of sterol biosynthesis inhibitors on growth and fine structure of Giardia lamblia P1 strain cultures were analyzed. Azasterols demonstrated high efficacy in killing cells. The IC50 values for 22,26-azasterol and 24(R,S),25-epiminolanosterol were 7 μM and 170 nM, respectively. Morphological analysis showed that azasterols induced changes in G. lamblia ultrastructure. The most significant alterations were: (a) considerable increase of the size of the peripheral vesicles, which are part of the parasite endosomal-lysosomal system; (b) appearance of autophagosomal structures; and (c) induction of differentiation, followed by an abnormal enlargement of encystation secretory vesicles. We propose that azasterols are effective chemotherapeutic drugs against Giardia lamblia in vitro and may have another target in cells besides sterol biosynthesis.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 363, Issue 2, 16 November 2007, Pages 310–316
نویسندگان
, , , , , ,