کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1937195 | 1050711 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acetylcholine induces neurite outgrowth and modulates matrix metalloproteinase 2 and 9
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کلمات کلیدی
PBSCCHDMEMFCSMMPACh - آهEDTA - اتیلن دی آمین تترا استیک اسید ethylene-diaminetetraacetic acid - اتیلن دیامین تتراستیک اسیدAcetylcholine - استیل کولینNeuronal differentiation - تمایز نورونfetal calf serum - سرم گوساله جنینPhosphate buffered saline - فسفات بافر شورmatrix metalloproteinase - ماتریکس متالوپروتئینازDulbecco’s modified eagle’s medium - محیط عقاب اصلاح شده DulbeccoChAT - چتCarbachol - کارباکولcholine acetyltransferase - کولین استیل ترانسفراز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Acetylcholine induces neurite outgrowth and modulates matrix metalloproteinase 2 and 9 Acetylcholine induces neurite outgrowth and modulates matrix metalloproteinase 2 and 9](/preview/png/1937195.png)
چکیده انگلیسی
The matrix metalloproteinases (MMPs), responsible for the degradation of extracellular matrix (ECM) proteins, may regulate brain cellular functions. Choline acetyltransferase (ChAT) transfected murine neuroblastoma cell line N18TG2, that synthesize acetylcholine and show enhancement of several neurospecific markers (i.e., sinapsin I, voltage gated Na+ channels, high affinity choline uptake) and fiber outgrowth, were studied for the MMP regulation during neuronal differentiation. Zymography of N18TG2 culture medium revealed no gelatinolytic activity, whereas after carbachol treatment of cells both MMP-9 and activated MMP-2 forms were detected. ChAT-transfected clone culture medium contains three MMP forms at 230, 92, and 66Â kDa. Carbachol treatment increased MMP-2 and MMP-9 gene expression in N18TG2 cells and higher levels for both genes were also observed in ChAT transfected cells. The data are consistent with the hypothesis that acetylcholine brings about the activation of an autocrine loop modulating MMP expression.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 362, Issue 2, 19 October 2007, Pages 269-274
Journal: Biochemical and Biophysical Research Communications - Volume 362, Issue 2, 19 October 2007, Pages 269-274
نویسندگان
Tonino Anelli, Ferdinando Mannello, Monica Salani, Gaetana A. Tonti, Giancarlo Poiana, Stefano Biagioni,