کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937240 1050711 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interface peptide of Alzheimer's amyloid beta: Application in purification
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Interface peptide of Alzheimer's amyloid beta: Application in purification
چکیده انگلیسی
Protein precipitation is a process commonly observed during bacterial expression of heterologous proteins. The high concentration of currently used solubilizing agents limits the scope of purification procedures. Protein solubilizers acting at very low concentrations will allow function-based purification protocols. Such applications are possible where sequence dependent protein-protein interactions occur. Oligomerization and higher order structure formation by proteins are examples where the interface peptide sequences are inhibitors of such interactions. In this study, we show that a partial sequence of Alzheimer's amyloid beta (Aβ) peptide can inhibit the aggregation. This enables the purification of protein containing Aβ sequence.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 362, Issue 2, 19 October 2007, Pages 538-542
نویسندگان
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