کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1937324 | 1050714 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Bidirectional membrane molecule transfer between dendritic and T cells Bidirectional membrane molecule transfer between dendritic and T cells](/preview/png/1937324.png)
The acquisition of dendritic cell (DC) molecules by T cells has been previously reported. However, it remains unclear whether the transfer is only mono- or bidirectional. In this study, we incubated CMFDA-labeled ovalbumin (OVA)-pulsed DC2.4 (DC2.4OVA) cells with Dil-labeled OT II CD4+ T cells and analyzed the potential bidirectional molecule transfer. We also assessed the distribution of internalized membrane using two engineered DC2.4/IabGFP and MF4/TCRCFP DC lines. Our findings showed that membrane molecule transfer is bidirectional. CD4+ T cells acquired Iab, CD11c, CD40, and CD80 from DC2.4OVA cells, and conversely DC2.4OVA cells took up CD4, CD25, CD69, and T cell receptor from T cells. The internalized molecules acquired by T cells and DCs mostly localized in endosomes and lysosomes, respectively. Taken together, this study demonstrated a novel phenomenon of bidirectional membrane molecule transfer between DCs and T cells.
Journal: Biochemical and Biophysical Research Communications - Volume 359, Issue 2, 27 July 2007, Pages 202–208