کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937433 1050716 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Observation of multiple intermediates in α-synuclein fibril formation by singular value decomposition analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Observation of multiple intermediates in α-synuclein fibril formation by singular value decomposition analysis
چکیده انگلیسی

One of the most well known characteristics for Parkinson’s disease (PD) is a polymerization of wild-type or mutant α-synuclein into aggregates and fibrils, commonly observed as Lewy bodies and Lewy neuritis in PD patients. Although numerous studies on α-synuclein fibrillation have been reported, the molecular mechanisms of aggregation and fibrillation are not well understood yet. In the present study, structural properties and propensities to form fibrils of wild-type, A30P, E46K, and A53T α-synucleins were investigated using fluorescence and circular dichroism (CD) methods. The results from these studies were analyzed using singular value decomposition (SVD) method which estimates a number of conformationaly independent species for a given process. The time-dependent CD spectra of the wild-type α-synuclein indicated a multi-step process in the fibril formation, and SVD analysis using the time-dependent CD spectra revealed that five or nine intermediates were formed at the early stage of fibrillation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 355, Issue 2, 6 April 2007, Pages 398–403
نویسندگان
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