کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1937761 | 1050725 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of exchange of amino acid residues of the surface region of the PST-01 protease on its organic solvent-stability Effect of exchange of amino acid residues of the surface region of the PST-01 protease on its organic solvent-stability](/preview/png/1937761.png)
The PST-01 protease from an organic solvent tolerant Pseudomonas aeruginosa has high stability and activity in the presence of various organic solvents. The structure gene of the PST-01 protease was amplified by the error-prone PCR method. The mutated proteases were incubated in the presence of acetonitrile. By measuring remaining activities, two kinds of mutated PST-01 proteases of which the stabilities were changed were selected. These mutations hardly changed the profile of the activity and stability at various pHs. Their activity and stability at higher temperatures were slightly lower than those of the wild-type PST-01 protease. The stabilities of the mutated enzymes in the presence of various organic solvents were greatly reduced. In both the mutated PST-01 proteases, amino acids located at the surface of the enzyme had been substituted.
Journal: Biochemical and Biophysical Research Communications - Volume 358, Issue 4, 13 July 2007, Pages 1028–1033