کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937765 1050725 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluid shear stress enhances the sphingosine 1-phosphate responses in cell–cell interactions between platelets and endothelial cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Fluid shear stress enhances the sphingosine 1-phosphate responses in cell–cell interactions between platelets and endothelial cells
چکیده انگلیسی

Fluid shear stress modulates the functional responses of platelets and vascular cells, and plays an important role in the pathogenesis of vascular disorders, including atherosclerosis and restenosis. Since shear stress induces activation of platelets, which abundantly store sphingosine 1-phosphate (Sph-1-P), and upregulates the mRNA expression of S1P1, the most important Sph-1-P receptor expressed on the endothelial cells, we examined the effects of shear stress on the Sph-1-P-related responses involving these cells. Shear stress was found to induce Sph-1-P release from the platelets in a shear intensity- and time-dependent manner. Inhibitors of protein kinase C suppressed this mechanical force-induced Sph-1-P release, suggesting involvement of this kinase. On the other hand, in vascular endothelial cells, shear stress increased S1P1 protein expression, as revealed by flow-cytometric analysis, and the responsiveness to Sph-1-P, which was assessed by monitoring the intracellular Ca2+ concentration. These results indicate that shear stress enhances the Sph-1-P responses in cell–cell interactions between platelets and endothelial cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 358, Issue 4, 13 July 2007, Pages 1054–1057
نویسندگان
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