کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1937781 | 1050725 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Characterization of two temperature-inducible promoters newly isolated from B. subtilis
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Here, two temperature sensitive promoters, P2 and P7, isolated from Bacillus subtilis, were characterized. The production of β-galactosidase driven by these promoters was much higher at 45 °C than that at 37 °C both in Escherichia coli and B. subtilis and that the P2 promoter showed higher expression strength in B. subtilis at 45 °C. Thereby, an efficient temperature-inducible expression system was constructed by using P2 promoter in B. subtilis. Thus, we isolated and characterized a newly temperature inducible promoter and exploited it as a potential expression element in B. subtilis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 358, Issue 4, 13 July 2007, Pages 1148–1153
Journal: Biochemical and Biophysical Research Communications - Volume 358, Issue 4, 13 July 2007, Pages 1148–1153
نویسندگان
Wang Li, Heng-Xin Li, Sheng-Yue Ji, Shuang Li, Yue-Sheng Gong, Ming-Ming Yang, Yu-Lin Chen,