کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1937794 1050726 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Simple screening method for differentially methylated regions of the genome using a small number of cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Simple screening method for differentially methylated regions of the genome using a small number of cells
چکیده انگلیسی
Genomic DNA methylation is a major epigenetic mechanism controlling the expression of genetic information. Therefore, identifying regions of the genome that are differentially methylated in different cells is a useful strategy for the study of biological phenomena. To date, several useful screening methods have been established for identifying differentially methylated genomic regions. However, it is impossible to use these methods in fields of study in which it is difficult to obtain a large number of uniform cells, because considerable amounts of genomic DNA are required. Given this situation, we developed a method for preparing large genomic DNA from a small number of cells, and a simple and highly sensitive method for screening for differentially methylated sites. Combined, these two methods comprise a simple screening method, which we named “Differential Methylation Site Scanning” (DMSS), for identifying differentially methylated regions of the genome from a small number of cells. Just 10 cells are sufficient for the method described here.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 353, Issue 2, 9 February 2007, Pages 275-279
نویسندگان
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