کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938137 1050734 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Analysis of HIF-prolyl hydroxylases binding to substrates
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Analysis of HIF-prolyl hydroxylases binding to substrates
چکیده انگلیسی

Hypoxia inducible transcription factors (HIF) are mainly regulated by a group of proline hydroxylases (EGLNs) that, in the presence of oxygen, target HIF for degradation. HIFα contains two independent oxygen degradation domains (N-ODD and C-ODD) that are substrates for these enzymes. In this work, we employed the yeast two-hybrid assay to study the sequence determinants required for the binding of EGLN1 and 3 to HIF1α in a cellular context. Our results demonstrate that, while EGLN1 is able to recognize both ODDs within full length HIF1α protein, EGLN3 only binds to CODD. The analysis of the residue substitutions within CODD uncovered novel critical determinants for EGLN1 and 3 binding. In addition, our results show that both enzymes have a very similar, albeit not identical, residue preference at specific positions in their substrate sequences.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 351, Issue 2, 15 December 2006, Pages 313–320
نویسندگان
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