کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938164 1050734 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Effects of hydrogen sulfide on homocysteine-induced oxidative stress in vascular smooth muscle cells
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Effects of hydrogen sulfide on homocysteine-induced oxidative stress in vascular smooth muscle cells
چکیده انگلیسی

Hydrogen sulfide (H2S) is an important gasotransmitter that generated in mammalian cells from l-cysteine metabolism. Little is known about its protective role in oxidative stress. In the present study, we investigated whether H2S could affect homocysteine (HCY)-induced cytotoxicity and oxidative stress in vascular smooth muscle cells. Cultured A-10 cells were exposed to HCY treatment in the presence or absence of NaHS (donor of H2S). HCY induced cytotoxicity, increased levels of H2O2, ONOO−, and O2- in a time- and concentration-dependent manner. Low levels of NaHS (30 or 50 μM) protected A-10 cells from cytotoxicity, decreased the production of H2O2, ONOO−, and O2- in the presence of HCY. Furthermore, NaHS enhanced inhibitory effects of NAC, GSH, DPI, SOD, L-NAME, or vitamin C on oxidized DCF or O2- formation induced by HCY. In conclusion, our findings provide the first evidence that low levels of H2S decrease reactive oxygen species and improve cell viability and by doing so limit cellular damage induced by HCY.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 351, Issue 2, 15 December 2006, Pages 485–491
نویسندگان
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