کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1938206 | 1050735 | 2006 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Detection of cholesterol-rich microdomains in the inner leaflet of the plasma membrane Detection of cholesterol-rich microdomains in the inner leaflet of the plasma membrane](/preview/png/1938206.png)
The C-terminal domain (D4) of perfringolysin O binds selectively to cholesterol in cholesterol-rich microdomains. To address the issue of whether cholesterol-rich microdomains exist in the inner leaflet of the plasma membrane, we expressed D4 as a fusion protein with EGFP in MEF cells. More than half of the EGFP-D4 expressed in stable cell clones was bound to membranes in raft fractions. Depletion of membrane cholesterol with β-cyclodextrin reduced the amount of EGFP-D4 localized in raft fractions, confirming EGFP-D4 binding to cholesterol-rich microdomains. Subfractionation of the raft fractions showed most of the EGFP-D4 bound to the plasma membrane rather than to intracellular membranes. Taken together, these results strongly suggest the existence of cholesterol-rich microdomains in the inner leaflet of the plasma membrane.
Journal: Biochemical and Biophysical Research Communications - Volume 351, Issue 3, 22 December 2006, Pages 713–718