کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938469 1050740 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Requirement of duplicated operons for maximal metabolism of phthalate by Rhodococcus sp. strain DK17
چکیده انگلیسی

The operons encoding the transformation of phthalate to protocatechuate are duplicated and present on two different megaplasmids [pDK2 (330 kb) and pDK3 (750 kb)] in Rhodococcus sp. strain DK17. RT-PCR experiments using gene-specific primers showed that both the pDK2- and the pDK3-encoded dihydroxyphthalate decarboxylase genes are simultaneously expressed during growth on phthalate. The doubling time of the pDK2-cured mutant strain DK176 in minimal liquid medium with 5 mM phthalate is 52.5% of that of the wild-type strain DK17. The data indicate that both copies of the phthalate operon are equally functional in DK17, and gene dosage is the main reason for slower growth of DK176 on phthalate.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 357, Issue 3, 8 June 2007, Pages 766–771
نویسندگان
, , , , ,