کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938521 1050741 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A new method for quantifying residue conservation and its applications to the protein folding nucleus
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
A new method for quantifying residue conservation and its applications to the protein folding nucleus
چکیده انگلیسی

The conservation of residues in columns of a multiple sequence alignment (MSA) reflects the importance of these residues for maintaining the structure and function of a protein. To date, many scores have been suggested for quantifying residue conservation, but none has achieved the full rigor both in biology and statistics. In this paper, we present a new approach for measuring the evolutionary conservation at aligned positions. Our conservation measure is related to the logarithmic probabilities for aligned positions, and combines the physicochemical properties and the frequencies of amino acids. Such a measure is both biologically and statistically meaningful. For testing the relationship between an amino acid’s evolutionary conservation and its role in the Φ-value defined protein folding kinetics, our results indicate that the folding nucleus residues may not be significantly more conserved than other residues by using the biological-relevance weighted statistical scoring method suggested in this paper as an alternative to entropy-based procedures.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 351, Issue 4, 29 December 2006, Pages 1031–1036
نویسندگان
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