کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938658 1050744 2007 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Biochemical characterization of the type I inositol polyphosphate 4-phosphatase C2 domain
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Biochemical characterization of the type I inositol polyphosphate 4-phosphatase C2 domain
چکیده انگلیسی
Inositol polyphosphate 4 phosphatases (IP4Ps) are enzymes involved in the regulation of phosphoinositide 3-kinase lipid signaling. They catalyze the hydrolysis of the 4-position phosphate from phosphatidylinositol 3,4-bisphosphate to phosphatidylinositol 3-phosphate. In this paper we have characterized a lipid binding C2 domain located on the N-terminus of type I IP4Ps. Mutational analysis of the lipid binding loops suggests that Asp61, Asp120, Asp123, Lys122, Arg124 are involved in lipid binding in vitro. In addition, we show that this C2 domain binds calcium but calcium is not involved in lipid binding. This paper provides insight into the mechanism of membrane interaction of IP4Ps.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 356, Issue 1, 27 April 2007, Pages 255-259
نویسندگان
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