کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1938661 | 1050744 | 2007 | 6 صفحه PDF | دانلود رایگان |

The bean (Phaseolus vulgaris) stress-related gene number 2 (PvSR2) is heavy metal-inducible. Here, the intron of PvSR2 (I-PvSR) within the coding sequence was isolated and characterized. I-PvSR exhibited a weak and constitutive promoter activity and enhanced the PvSR2 promoter activity in transiently transformed tobacco protoplasts. The transcription start site of I-PvSR promoter was mapped 72 bp upstream of the 3′-splice site. The shorter PvSR2 transcript (768 nt) in bean is generated from this intronic promoter and lacks the last 56 bases of 3′-end sequence of longer PvSR2 transcript (829 nt) by utilizing an alternative polyadenylation site. Quantitative competitive PCR analysis further revealed that two transcripts were differently accumulated in response to Hg2+-exposure and the longer transcript was more abundant than the shorter one. These results demonstrate an additional non-metal inducible transcription of PvSR2 via alternative intronic promoter usage and provide new insights into expression mechanism of metal inducible gene.
Journal: Biochemical and Biophysical Research Communications - Volume 356, Issue 1, 27 April 2007, Pages 273–278