کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938818 1050747 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Shift syndecan-2 from RACK1 to caveolin-2 upon transformation with oncogenic ras
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Shift syndecan-2 from RACK1 to caveolin-2 upon transformation with oncogenic ras
چکیده انگلیسی

Syndecan-2 was found to detach from RACK1 and associate with caveolin-2 and Ras in cells transformed with oncogenic ras. Most of syndecan-2 from transformed cells was revealed with negligible phosphorylations at tyrosine residues. We experimented with HeLa cells transfected with plasmids encoding syndecan-2 and its mutants (syndecan-2Y180F, syndecan-2Y192F, and syndecan-2Y180,192F) to provide evidences that PY180 of syndecan-2 is a binding site for RACK1 and is deprived in cells transfected with oncogenic ras. However, in HeLa cells transfected with syndecan-2Y180F, RACK1 was found to sustain its reactions with syndecan-2 independent of phosphorylation. The finding of syndecan-2 reactive with caveolin-2/Ras suggests the molecular complex most likely to obstruct RACK1 for functional attachment at syndecan-2, as revealed in cells transfected with oncogenic ras. We provided evidences to reinforce the view that molecular rearrangements upon transformation are specific and interesting.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 350, Issue 1, 10 November 2006, Pages 227–232
نویسندگان
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