کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1938881 1050749 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Novel biomimetic affinity ligands for human tissue plasminogen activator
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Novel biomimetic affinity ligands for human tissue plasminogen activator
چکیده انگلیسی

Dyes-based biomimetic affinity chromatography has been used to purify therapeutically useful proteins. In order to design novel biomimetic affinity ligands for purification of tissue-type plasminogen activator (t-PA), small molecular fragments were achieved to fit in S3/4 binding site of t-PA by structure-based ligand design method (InsightII/Ludi). Three biomimetic affinity ligands A, B, and C were then designed, synthesized, and proved to bind the target protein (t-PA), exceeding the binding capacity of the commercial p-amino benzamidine affinity matrix. The designed affinity matrix A showed high efficiency to purify sc-tpa from the crude samples with 18-fold of purification.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Biochemical and Biophysical Research Communications - Volume 355, Issue 3, 13 April 2007, Pages 673–678
نویسندگان
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