کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1938919 | 1050750 | 2006 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Alternative splicing variants of IRF-1 lacking exons 7, 8, and 9 in cervical cancer Alternative splicing variants of IRF-1 lacking exons 7, 8, and 9 in cervical cancer](/preview/png/1938919.png)
The two previously identified major splice variants of interferon regulatory factor 1 (IRF-1) do not appear to affect IRF-1-mediated gene activation. We searched for additional splice variants and examined their effect on wild-type IRF-1. RT-PCR experiments using normal and malignant human cervical tissue samples revealed five variants lacking some combination of exons 7, 8, and 9; their expression levels were higher in the malignant samples. These variants had predicted deletions of the functional domain or truncated protein isoforms, had different transcriptional activities, and attenuated transcriptional activity of IRF-1. Unlike the cell cycle-dependent IRF-1 transcript, the splice variant mRNA levels remained consistent throughout the cell cycle. The variant proteins were more stable than the IRF-1 protein, which may explain the strong inhibition of IRF-1 transcription in the presence of relatively small quantities of the alternative transcripts. In conclusion, alternative splicing in exons 7, 8, and 9 is an important mechanism for negatively regulating IRF-1 in cervical cancer.
Journal: Biochemical and Biophysical Research Communications - Volume 347, Issue 4, 8 September 2006, Pages 882–888